Research has been ongoing since the 1960s to determine what the proportion of in the atmosphere has been over the past fifty thousand years.The resulting data, in the form of a calibration curve, is now used to convert a given measurement of radiocarbon in a sample into an estimate of the sample's calendar age.Animals eat the plants, and ultimately the radiocarbon is distributed throughout the biosphere.
Libby and James Arnold proceeded to test the radiocarbon dating theory by analyzing samples with known ages.For example, two samples taken from the tombs of two Egyptian kings, Zoser and Sneferu, independently dated to 2625 BC plus or minus 75 years, were dated by radiocarbon measurement to an average of 2800 BC plus or minus 250 years. Carbon dioxide produced in this way diffuses in the atmosphere, is dissolved in the ocean, and is taken up by plants via photosynthesis.the average or expected time a given atom will survive before undergoing radioactive decay. The calculations involve several steps and include an intermediate value called the "radiocarbon age", which is the age in "radiocarbon years" of the sample: an age quoted in radiocarbon years means that no calibration curve has been used − the calculations for radiocarbon years assume that the , which for more than a decade after Libby's initial work was thought to be 5,568 years.This was revised in the early 1960s to 5,730 years, which meant that many calculated dates in papers published prior to this were incorrect (the error in the half-life is about 3%).Other corrections must be made to account for the proportion of throughout the biosphere (reservoir effects).
Additional complications come from the burning of fossil fuels such as coal and oil, and from the above-ground nuclear tests done in the 1950s and 1960s.The radiocarbon dating method is based on the fact that radiocarbon is constantly being created in the atmosphere by the interaction of cosmic rays with atmospheric nitrogen.The resulting radiocarbon combines with atmospheric oxygen to form radioactive carbon dioxide, which is incorporated into plants by photosynthesis; animals then acquire in a sample from a dead plant or animal such as a piece of wood or a fragment of bone provides information that can be used to calculate when the animal or plant died. The method was developed by Willard Libby in the late 1940s and soon became a standard tool for archaeologists.Libby received the Nobel Prize for his work in 1960.By contrast, methane created from petroleum showed no radiocarbon activity because of its age.