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Wild-type Sindbis utilized in these studies was generated from plasmid p Toto1101 (9).5); ns P1 is important for capping the RNAs and interacts with membranes, ns P2 contains the aforementioned proteolytic activity, exhibits nucleosidetriphosphatase and helicase activities, and is involved in regulation of RNA synthesis.

While known to be essential for viral replication, the functions of ns P3 are not yet understood.

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